In conclusion, Serial Cloner is a very user-friendly and powerful Molecular biology tool. Fragments obtained by PCR, shRNA synthesis, can be easily assembled by selecting between the restriction sites. ![]() When using the Installer, simply run it and follow the instruction. Erase previous versions of Serial Cloner. We can also ligate fragments using the ligate button To Install Serial Cloner (Mac and Windows): When using the archive, download the file (Disk image for MacOSX or compressed Zipped file For Window, un-compress it and copy the resulting Serial Cloner Folder in your Application folder.Also, to create new PCR based fragments or synthetic adaptors. We can use either the graphics window or the text-based site usage windows, which makes it very easy to quickly extract a sub-sequence in a selection or in any restriction sites.There is a web browser tool within the software to allow direct import of NCBI and EMBL.Sequence features are visible on the sequence map.They can be automatically found by using the scan feature. Features can be manually entered or imported.The fully graphical map makes it easy to extract and select a fragment or even show single, double, or multiple cutters in a single window.All the tools required to analyze the sequence and modify if any changes needed are available in a single window making it very easy to use. ![]() The software comes with a high-quality graphics display and has a very user-friendly interface to the front end, which makes the interface very intuitive. They are processed both in sequence and as a graphic map. ![]() Since version 2.0 of the software, it also handles Annotations and features. It can read and write files in several formats, making it portable across multiple users who use different Molecular Biology tools. It is available free for download and can be used across multiple Operating Systems, including Windows, Mac, and Linux. Serial Cloner is a Molecular Biology Tool which is a front end to help with DNA Cloning, Sequence Analysis, and Visualization.
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![]() 1,2 A low percentage of these disorders present without the production of any monoclonal protein. These disorders are commonly characterized by the production of monoclonal proteins which may be either intact immunoglobulins (M-Ig), serum free light chains (sFLC), a combination of both, or rarely, free heavy chains only. Monoclonal Gammopathies (MGs) include premalignant Monoclonal Gammopathies of Uncertain Significance (MGUS), Smoldering/Indolent Multiple Myeloma and malignant conditions. The aim of this article is to provide a clear discrimination between the two assays and to provide information to physicians and laboratory technicians so that they can utilize the International Myeloma Working Group guidelines. Furthermore, we elaborate on our study comparing the two assays used in 11 Light Chain Multiple Myeloma patients at presentation and 103 patients taken through the course of their disease. This paper reviews the available literature comparing the two assays and tries to clarify hypothetical limitations of the total assay to detect Multiple Myeloma. Other assays that measure light chains in the free and intact immunoglobulin forms are sensitive, but unfortunately, due to the nomenclature used, these assays (total light chains) are sometimes used in place of the free light chain assay. The serum free light chain assay recognizes the light chain component of the immunoglobulin in its free form with high sensitivity. Since 2006, the serum free light chain test Freelite ® has been included alongside standard laboratory tests (serum and urine protein electrophoresis, and serum and urine immunofixation) as an aid in the identification of monoclonal proteins, which are a cornerstone for the diagnosis of Multiple Myeloma. However, early diagnosis is associated with less severe disease, including fewer patients presenting with acute renal injury, pathological fractures and severe anemia. The diagnosis of Multiple Myeloma is a challenge to the physician due to the non-specific symptoms (anemia, bone pain and recurrent infections) that are commonplace in the elderly population. ![]() Looking for an alternative or similar product to the Steam digital gift card? We recommend: That's what we call fast, safe and simple. You then get your Steam gift card Australia code by email within 30 seconds. Here's how it works: select the amount of the Steam gift cards you want and pay with PayPal, VISA, MasterCard (credit card/debit card), UnionPay, or more than 23 other safe and secure payment methods. To ensure you don't miss out and get yourself a Steam card on ! Redeem it to recharge your Steam wallet card and enjoy the best deals in the world of PC gaming. Great new indie games are also coming out every week. It's not uncommon to see awesome AAA games up to 50% off. Who needs an expensive console when you can play the best games on your PC with a Steam gift card? Join the Steam gaming community and get access to great new PC games and exclusive Steam sales. You can see all the options on our Gaming Card page. Looking for an alternative or similar product to Steam Gift Cards? We recommend: ![]() You then get your Steam digital Gift Card code by email within 30 seconds. Here's how it works: select the amount of the Steam Voucher you want and pay with PayPal, Mastercard, Visa (credit card/debit card), Apple Pay or with more than 23 other safe and secure payment methods. Redeem it to recharge your Steam wallet and enjoy the best deals in the world of PC gaming. To ensure you don't miss out and get yourself a digital Steam Card on. Who needs an expensive console when you can play the best games on your PC with a Steam Gift Card? Join the Steam Australia (AU) gaming community and get access to great new PC games and exclusive Steam sales with Steam Cards from. ![]() You can save each item in the clipboard and access it later. It allows users to access copied items such as text, files, and images it is free to use and the copied item is automatically saved and accessed by right-clicking it is very reliable and stress-free.ĭitto is a standard Windows clipboard extension. ClipXĬlipx is one of the many variants of the windows clipboard manager it is very easy to use and one of the best. It provides the functionality to edit, change the format, and manage the items stored in the clipboard. This feature also saves time and makes you more efficient by adding clipboard functions that Windows Clipboard eliminates, such as the ability to store as many clips as you want instead of just one. Must Read: How To Use WhatsApp, Telegram, and Other Messengers on Chrome This happens in situations when you’re not sure whether to delete it or accidentally delete a copied item or file. Clip MateĬlip mate has lots of cool features pertaining to the clipboard, What’s amazing about it is that if you delete an item from the clipboard, it moves the deleted item to the recycle bin instead of discarding it. This is a very practical and easy application that can be used as a search tool. Clip Angel also stores and displays the copied URLs. It minimizes and pastes items to the default application automatically. The icon is displayed for the file type that is copied. The best thing about it is that you can store everything you copy such as texts, files, and folders. ![]() Clip AngelĬlip Angel is one of the best clipboard administrators that can be used on Windows computers and is free. Windows on default come with a clipboard manager, however, the fact that you are reading this article shows that the default provision doesn’t meet all your needs, hence you have sought to seek third-party provisions. In summary, Clipboard managers help with cut, copy and paste functionality. The reason behind the ubiquitousness of Clipboard managers lies in its imperativeness, as it offers enthusiasts the ability to hold copied items in a retrievable space, which means you will be able to copy items from point A and dump them in Point B. The reason behind this development is actually not far-fetched. For winBest Clipboard Managers For Windows PC: The technology of clipboard managers has over the years found its way into the different operating systems, with the Windows OS being inclusive. Jean-Claude Risset, the French composer of computer-based music, first pioneered the use of a Shepard Scale that moves upwards or downwards in continuous pitch as opposed to discrete half-steps. The Shepard Scale The Shepard-Risset Glissando The illusion also works in the reverse if one wishes to create a never-ending descending scale. This creates the illusion of a never-ending ascending scale, much like a barber pole, because one never hears the absolute end.Īs one line of the higher pitches fades out, the ear is drawn to one of the lower lines, which continues its ascent until it too fades out. The Shepard “tones” that are lowest in pitch fade in gradually as the scale ascends, and then fade out as they reach the peak frequency. The Shepard Scale is essentially a chromatic scale made up of Shepard Tones. But just by listening to a static Shepard Tone, the familiar illusion is not apparent. A Shepard Tone built on C includes every C from C-2 to C8īecause the Shepard Tone is created using pure sine waves, its spectrograph image indicates a sharp peak at every frequency included from its lowest octave iteration to its highest octave iteration.Įach of these tones is fundamental in the Shepard Tone tone profile and there are no partials along the way. In Ableton Live, for example, you can open Operator (which defaults to a sine wave), and proceed to input every iteration of a particular pitch class, covering the entire range of human hearing.Īs illustrated below in the case of a Shepard tone constructed on C, the MIDI roll will be composed of every C from C -2 to C 8. One simply loads up their DAW of choice and opens a basic synthesizer. While it required some truly innovative computing at the time of its origin, creating a Shepard Tone is fairly simple in the present day. How Do You Make A Shepard Tone In Ableton Live? These did not exist prior to electronic synthesis, which is why the Shepard Tone only came to be in the 1960s. ![]() ![]() The most essential building blocks of the Shepard Tone are pure sine waves. These terms tend to get used interchangeably which can make things confusing. In this case, the Shepard Tone has been “animated” to create a Shepard Scale – the illusion you are probably already familiar with. If you’ve looked up examples of Shepard Tones, you’ve probably heard a sound that seems to move either up or down but never reaches its destination. ![]() How Is A Shepard Tone Created?įirst, we need to distinguish between a Shepard Tone and a Shepard Scale. In this article, we will explore the Shepard Tone and its uses for both music-related research as well as composition and production. While tones played on instruments consist of a fundamental pitch and a series of softer partials, the Shepard Tone essentially has no single fundamental, and any partials are equal in volume.ĭue to this, the Shepard Tone allows one to engage with pitch as if all octaves were flattened into a single set of twelve pitch classes that seem to move up or down infinitely, like an auditory barber pole. The Shepard Tone is named after Roger Shepard, the cognitive scientist who first generated Shepard Tones and published his research in a 1964 article published in The Journal of the Acoustical Society of America entitled Circularity in Judgments of Relative Pitch. ![]() Put most simply, the Shepard Tone is a computer-generated tone consisting of every octave of a single pitch superimposed into a single sound. Also consider checking out our guide on the tritone paradox.What are some examples of the Shepard Tone in music and sound design?.How has the Shepard Tone been used for research in music perception?.Disclosure: We may receive commissions when you click our links and make purchases. OXENFREE II: Lost Signals follows the story of Riley Poverly, an environmental researcher who returns to her hometown Camena to investigate unnaturally occurring radio frequency signals causing disturbances in electronic equipment. Players will have to determine every aspect of Alex’s story while exploring Edwards Island, maneuvering strange events, eccentric peers, ominous creatures and more in order to save their souls and close the rift that was accidentally created. ![]() The night takes a terrifying turn when the group of friends unwittingly opens a ghostly rift spawned from the island’s cryptic past. In OXENFREE, players take on the role of Alex, a bright, rebellious teenager who brings her new stepbrother Jonas to an overnight party on Edwards Island, an abandoned military island. You can learn more about OXENFREE and OXENFREE II: Lost Signals by visiting, and following Night School Studio on Twitter, Facebook, and Instagram. You can also check out the latest tape, “ Looping back to OXENFREE”, in a series of videos promoting the upcoming launch of OXENFREE II: Lost Signals, in which the developers dive into the not-so-linear timeline of the game, while also looking back on the original OXENFREE game. The game is also available for Netflix subscribers as part of their membership. The mind-bending follow-up to the critically-acclaimed narrative adventure game OXENFREE follows Riley Poverly as she revisits her childhood to investigate haunting signals but finds something much bigger than she bargained for.įor players yet to dive into OXENFREE’s supernatural adventure can rejoice as the original game is available to purchase for 80% off on PC or Mac via Steam, the Nintendo Switch, and 75% off on PlayStation 4 for a limited time. Today OXENFREE II: Lost Signals launches for PC and Mac via Steam, PlayStation 4 and PlayStation 5, Nintendo Switch and Netflix. ![]() Players can also get OXENFREE at a discounted price on Steam, PlayStation, and Nintendo Switch These ones are faster, more nimble but also harder to control. ![]() For experienced pilots, they tend to use smaller but heavier gliders. The gliders that we will be using are bigger, slower, and first-timer friendly. When it is a bigger group (max 8 people), they run two gliders instead of one to keep the wait times down. As we wandered over to a glider sitting on top of the sand dune, we saw our awesome instructors Josh and Andy assembling a second glider. On the day of our adventure, we arrived about 30 minutes early since we were super excited. Josh is also a superhuman who can carry the hang glider up the 30 ft sand dune 201 times in one day! Josh is super enthusiastic and willing to teach all of his knowledge. However, since he started flying in 2008, he can’t get enough of it. Josh received his Bachelor’s in Aeronautics from Embry-Riddle Aeronautical University. Since Andy has been teaching for a long time, he is a well of flight knowledge. He’s also super funny and full of useful tips and tricks. Andrew and Josh are some of the most qualified hang gliding and tandem flight instructors out there.Īs far as being veteran goes, Andrew (Andy) has been flying for over 35 years. In 2018, Andrew Beem and Josh Laufer took over the company and have continued the tradition of flight excellence. Since then, Windsports has been providing training to new pilots as well as tourists in the Los Angeles area for over 40 years. These two pilots came together to teach the sport that they loved so much. Windsports was established in 1970 by Joe Greblo and Rich Grigsby. You need to get there at least 15 minutes early. Since it’s in Los Angeles, you can do this year-round. From the glider area, if you looking at the beach, it is on the right-hand side, down the walkway.ģ-4 hours depending on the size of the group The restroom is located downstairs at the beach cafe. Parking is $10 on weekdays, $13 on weekends. You can sign in there or go find the instructors near the gliders located right on top of the sand dunes) There is a small beige trailer located at the end of the parking lot. (Once you go into the parking lot, make a left after the ticketing booth, toward the candy-striped looking smokestacks. ![]() Check out their website for more up to date pricing.ĭockweiler State Beach – 12501 Vista Del Mar, Los Angeles, CA 90293 They also offer tandem flights up in the mountains. On our recent visit, we brought our fellow adrenaline-hungry adventurers with us.Ĭlasses: Windsports offers mini and full beach hang gliding lessons. We had so much fun that we’ve been wanting to come back ever since. ![]() So obviously, we immediately talked Dustin’s dad into taking the class with us. Flying has always been a passion for both of us. About 4 years ago, we were so excited to find out that we could take lessons for hang gliding in Los Angeles. The 2XS size is suitable for dogs with a snout girth of 6 1/2 inches and a length of 1 9/16 inches. This muzzle is perfect for preventing dog biting or eating off the ground, as well as for training, walking, grooming, and vet visits. This BronzeDog Wire Basket Dog Muzzle is designed for small dogs, such as Jack Russell Terriers, and is made out of high quality genuine leather and metal wire. ![]() With these tips in mind, you can make sure that your pup is comfortable and safe when wearing a muzzle. Additionally, opt for a muzzle that is durable and won’t rub or chafe your pup’s skin. Make sure to measure your pup’s snout, find a breathable and comfortable material, and look for one that won’t restrict your pup’s vision or breathing. When looking for the best muzzle for your Jack Russell Terrier, it is important to consider all of these factors. Design: Consider the design of the muzzle and make sure it won’t cause excessive rubbing or chafing. Durability: Look for a muzzle that is made of durable materials that won’t break easily.ĥ. Safety: Choose a muzzle that won’t restrict your pup’s breathing or vision.Ĥ. Size: Jack Russell Terriers come in a variety of sizes, so make sure to measure your pup’s snout to find the right sized muzzle.ģ. Look for muzzles that are made of breathable material, such as nylon or leather, and make sure they fit snuggly but not too tightly.Ģ. ![]() Comfort: Make sure the muzzle you choose is comfortable for your pup. Here are five key points to consider when looking for the best muzzle for your Jack Russell Terrier:ġ. But with the right information and knowledge, you can make sure that your pup is comfortable and safe. Finding the best muzzle for a Jack Russell Terrier can be a daunting task. Thank you is an understatement for everything you are doing. Thank you Coronavirus helpers from the bottom of our hearts. The Coronavirus helpers make us feel that someone is in control in this uncontrollable world. These lifesavers bring positivity each day and help make the world a better place. They are the real heroes who are saving lives each day in more ways than one. They are the ones who are changing our world with their bravery. When we thank the helpers, we thank all of them. Finally, a big thank you to our loved ones who connect with us through calls, texts, and more to check in on us and listen to our worries, thank you! Similarly, the police officers who are out on roads patrolling the area and making sure everyone is safe. In addition, the school staff who continue our children’s education. The delivery workers delivering products, mails, food items, medicine, and more are our saviours. ![]() They work to protect our parents, grandparents and more. Most importantly, a big thank you to the nursing home staff workers. The ones who create groups online so people in need can connect with each other and get local information. Thank you Coronavirus helpers like the community leaders who hand out essential items to the needy. thank you coronavirus helpers doodle poll So, a big thank you Coronavirus helpers for everything you’ve done and are still doing. Be it medical professionals or sanitation workers, all of them deserve a big thank you. While most of us stayed safe in our houses, the helpers stepped out to ensure our safety. But, another thing that was common throughout the world was the Coronavirus helpers. Everyone all over the world has suffered due to it in some way or the other. The Coronavirus pandemic has hit us hard. To express your appreciation and gratitude for these everyday heroes, visit /UChicagoMed and use #ThousandsofDifferences to share encouraging words, artwork and thank you notes. Your service to patients is saving countless lives and making thousands of differences. Your dedication, commitment and courage deserve our deepest gratitude and admiration. Thank you - from everyone at UChicago Medicine and Ingalls Memorial - for the sacrifices you make, every day and especially during this pandemic. Proudly express your appreciation by downloading, printing and displaying the “Thank you to the helpers” sign below in the window of your home, business or vehicle. Join the movement to thank all the people – the grocery store workers, truck drivers, first responders, health care workers and more – who are making sacrifices to support the COVID-19 coronavirus fight. Today, we’d like to say: To all coronavirus helpers, thank you. We’re launching a Doodle series to recognize and honor many of those on the front lines. As COVID-19 continues to impact communities around the world, people are coming together to help one another now more than ever. ![]() Try a Different Whole Wheat Biscuit Recipe Since baking is a chemical reaction, even small differences in how ingredients react can have a negative effect on your baking. If this could be the issue, try making some adjustments for baking success.Īltitude is another issue for baking it can affect how baking powder reacts and how quickly liquids evaporate. Even if you’re not in a humid area, a string of rainy weather can have the same effect too. In areas with high humidity baking ingredients can draw moisture in from the air, which affects their performance. If you’ve tried everything and still can’t get your biscuits to rise your altitude or the weather might be to blame. This steam adds air between the biscuit layers which, along with the baking powder, helps biscuits rise.įor best results, make sure your butter has been chilled before adding it (straight out of the fridge), don’t let dough get too warm while you work it, and wait for the oven to preheat fully before baking your biscuits. When you place biscuits in the oven the cold butter heats up and produces steam. Mixing in an electric mixer or over kneading will make the dough rubbery, which doesn’t bake well. It’s important to knead the dough by hand as few times as possible when you make homemade biscuits. Good baking powder should activate and fizz when it hits the water. To test baking powder, add ¼ teaspoon of baking powder to ½ cup of hot water. This can be even shorter if the container isn’t airtight. Even if it’s not expired, baking powder loses its potency about 6 months after opening. Old Baking Powderįirst, check to make sure your baking powder hasn’t expired, especially if you don’t bake often. Here’s some of the most common reasons whole wheat biscuits don’t rise properly and what you can do to correct it. If you’re new to making homemade biscuits it might take a bit of practice to get a perfect light and fluffy texture. Other yummy real food biscuit spreads: Honey, natural peanut butter, homemade whipped cream, fruit, and butter. If you like biscuits and gravy: Swap out the traditional sausage and white flour gravy with homemade gravy made from roasting pan drippings.įor biscuits and jam: Use homemade jam or opt for 100% pure store bought varieties with no added refined sugar.īreakfast biscuit sandwich: Make a healthier version of this fast food breakfast at home with egg, cheese, and your choice of meat. This makes for a quick addition to dinner for those busy weeknights. You can throw the frozen biscuits in the microwave or toaster oven on the bake setting. The amount of baking powder in self-rising flour and this biscuit recipe may differ if you find your biscuits don’t rise with self-rising flour, add an additional teaspoon of baking powder to the mixture. You’ll be left with an easy 3 ingredient biscuit recipe! To make these biscuits with whole wheat self-rising flour, simply omit the baking powder and salt. Some people prefer it for southern style recipes like biscuits because it saves on prep time, and you don’t have to stock as many ingredients. Self-rising flour is just regular flour with baking powder and salt already added to it. Bake on an ungreased baking sheet for about 10-12 minutes, or until the tops of the biscuits are golden brown.Įasy 3 Ingredient Biscuit Recipe With Self-Rising Flour.Cut out circular shapes using a drinking glass upside down, or you can use a cookie cutter.Pat it flat on a floured surface so that it’s an even 3/4″ thickness.Knead the dough with your hands 8-10 times, but do not over-knead it.You can also use a stainless steel dough blender. It’s okay if the mixture just looks like tiny butter chunks covered in flour. Mash the butter pieces into the mixture using the back of a fork.Cut the butter into small pieces and mix it into the dry mixture.Combine all dry ingredients in a medium bowl and whisk with a fork.You can cut out your biscuits with cookie cutters or a drinking glass How to Make Whole Wheat Biscuits |
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